Inner Orbital Ground State Excited State Outer Orbital Excitation (light) Emission (light) Nucleus Electrons moving around the nucleus of atoms or molecules can be excited by light (they absorb light). Ĥ What is Fluorescence? Excitation (light) Emission (light) Presentation on theme: "Reading Fluorescence with a Microplate Reader BioTek Instruments, Inc."- Presentation transcript:ġ Reading Fluorescence with a Microplate Reader BioTek Instruments, Inc.Ģ Fluorescence FI- Fluorescence Intensity FP- Fluorescent Polarizationģ What is fluorescence? Reading fluorescence in a microplate Excitation and Emission parameters How to.
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